1. Introduction

    1. Defense of method
    2. Definitions and discussion of traditional genetics
    3. Definition and discussion of genetic engineering
    4. Basic molecular biology
    5. Recombination
    6. The genetic code

  2. Basic genetic methods

    1. Screens
    2. Enrichments
    3. Selections

  3. Mutation frequency

    1. Inherent Mutation frequency/definitions
    2. Chemical mutagens
    3. More mutagenesis
    4. DNA repair mechanisms
    5. Summary of mutagenesis
    6. Mutant detection

  4. Complex mutations

    1. Duplications
    2. Amplifications
    3. Inversions
    4. Deletions
    5. Mobile genetic elements
    6. in vitro mutagenesis requiring sequence/site information

  5. Effects of mutations

    1. Potential Effects of mutations
    2. Numerology

  6. Gene transfer systems
    1. Plasmids and conjugation
    2. Transformation
    3. Transduction

  7. Complementation

    1. Definition
    2. Complications

  8. Mapping

    1. Why do it? Definitions
    2. Two-Factor crosses
    3. Deletion mapping
    4. Other types of mapping
    5. Final notes on genetic mapping
    6. Strain construction

  9. Genetic engineering/inverse genetics

    1. Identification of interesting DNA fragments
    2. Further characterization of cloned regions
    3. Analysis and use of clones and sequences
    4. A general approach to the analysis of a bacterial system

  10. Fusions
    1. Definitions
    2. Utility
    3. Generation
    4. Controls/concerns

  11. Suppression

  12. Sample problems and answers

    1. Sample problems part 1
    2. Sample problems part 2