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Table of Contents
Why Do Genetics
Genetic Terms
More Terms
Basic Molelcular
Biology

More Basic Concepts
Screens
Selections
Mutation Frequency
Chemical Mutagenesis
Frameshift Mutation
DNA Repair
Mutation Summary
Detecting Mutants
Complex Mutation
Insertion Sequences
Compound Transposons
Complex Transposons
Models of
Transposition

Transposition Summary
Mutagenesis in vitro
Effects of Mutations
Complementation
Plasmids and
Conjugation

F Factor
Transformation
Transduction
Generalized
Transduction

Specialized
Transduction

Complementation
Mapping
Two Factor Crosses
Deletion Mapping
Other Mapping Methods
Strain Construction
Inverse Genetics
Gene Isolation
Characterization of
Clones

Sequence Data
General Approaches
Fusions
Supression
Final Summary
Problem Set 1
Problem Set 2


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Summary

©2000 written by Gary Roberts, edited by Timothy Paustian, University of Wisconins-Madison

SUMMARY

This text has attempted to point out both the strengths and weaknesses of genetic analyses. To summarize, the strengths of genetics are that

  1. It lets you search for, and demand, rare events;

  2. It can indicate, when a number of mutations in a given region are examined, the range of phenotypes and therefore the range of biochemical interactions that the gene product is involved in;

  3. It is done in vivo, in distinction to the in vitro analysis of most biochemical approaches.

The weaknesses of genetics are that

  1. The mutations necessarily perturb the system;

  2. It is necessarily indirect (for example, complementation groups are not synonymous with genes);

  3. It can take a significant number of time to "set up" the system for proper genetic analysis;

  4. Without biochemical characterization of the analyzed mutants, a genetic analysis lacks "functional interpretibility".

Thank you, I hope you found it useful, and good night everybody.

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